Sample must be cleaned and free of interferents. Compatible with both the QX100 and QX200 Droplet Digital PCR Systems. Polymerase Chain Reaction Fluorescence īIORAD - QX200 Auto Droplet Generator.pdf|BIORAD - QX200 Droplet Reader.pdf|ĭroplet Generator: 11 x 14 x 5 in (28 x 36 x 13 cm), Droplet reader: 26 x 20 x 11 in (66 x 52 x 29 cm) Generates droplets for 96 ddPCR reactions in under 45 minutes. The droplet generator is used with the QX200 droplet reader for EvaGreen or probe-based digital PCR applications. The fraction of PCR-positive droplets enables the target to be quantified according to the Poisson distribution. Use the QX200 droplet generator to complete the first step of the Droplet Digital PCR (ddPCR) workflow by partitioning ddPCR reaction mix into 20,000 nanoliter-sized droplets for up to 8 samples per run. Positive droplets, which contain at least one copy of the target DNR or RNA molecule, exhibit increased fluorescence compared to negative droplets. The droplets stream single file through the reader for fluorescence analysis. Droplets are transferred to a 96-well PCR plate and PCR is performed to end point in a thermal cycler. Use DG32 Automated Droplet Generator Cartridges to generate nanoliter-sized PCR reaction droplets with the QX600/QX200 AutoDG Droplet Digital PCR (ddPCR) System. Each droplet serves to partition the reactions. The QX200 droplet generator, which can process eight samples at a time and requires samples to be manually pipetted into sample wells, partitions each sample into 20,000 uniform nanoliter-sized droplets in which nucleic acid molecules are randomly distributed. The system consists of four instruments: a droplet generator, a plate sealer, a thermo cycler, and a droplet reader. droplets using an automated droplet generator, and the droplet emulsion is transferred into a 96 - well PCR plate for PCR amplification. Droplet generator (28 pages) Laboratory Equipment BIO RAD QX100 Instruction Manual. The Bio-Rad QX200 ddPCR System combines water-oil emulsion droplet technology with microfluidics. Droplet reader, ivd and quantasoft software (40 pages) Inverter Bio-Rad QX200 Instruction Manual.
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